Abstract: Background The diet is an important strategy for glycemic control as well as reducing/eliminating the risk of developing diabetes. Objective This article evaluated the consumption of macronutrients and micronutrients of pre-diabetic individuals from Santa Cruz do Sul, in the state of Rio Grande do Sul (RS), Brazil. Method The nutrients intake was determined in the DietWin® program and the evaluation of the prevalence of adequacy macronutrients was classified according to Guidelines of the Brazilian Society of Diabetes and the evaluation of inadequacy of the micronutrients intake by Dietary Reference Intake were used. Results The pre-diabetic individuals had excessive protein and total fat intake (28.1% and 31.6%, respectively), as well as saturated fatty acids and cholesterol (91.2% and 35.1%, respectively). The fibers intake in this population showed an inadequacy of 93.0%. Considering the micronutrients intakes, vitamin D, calcium and selenium presented an insufficiency greater than 80.0%. Conclusion The usual diet of pre-diabetic individuals showed to be excessive for proteins and total fats, which associated with excessive consumption of saturated fatty acids and cholesterol, can increase cardiovascular risk. This, coupled with inadequate consumption of some micronutrients such as vitamin D, calcium and selenium and low fiber can affect the control of glycemia in these individuals.

Resumen Introducción La alimentación es una importante estrategia para el control de la glucemia, así como para reducir/eliminar el riesgo del desarrollo de la diabetes. Objetivo Este artículo evaluó el consumo de macronutrientes y micronutrientes de individuos prediabéticos de Santa Cruz do Sul, RS, Brasil. Método El consumo de nutrientes se ha determinado en el programa DietWin® y la prevalencia de adecuación de los macronutrientes fue clasificada según la Sociedad Brasileña de Diabetes y la ingesta inadecuada de micronutrientes por la Dietary Reference Intake. Resultados Los prediabéticos presentaron consumo excesivo de proteínas y grasas totales (28,1% y 31,6%, respectivamente), así como de ácidos grasos saturados y colesterol (91,2% y 35,1%, respectivamente). La ingesta de fibras se presentó insuficiente en 93%. Considerando el consumo de micronutrientes, la vitamina D, el calcio y el selenio se presentaron con una insuficiencia de más del 80%. Conclusión La dieta habitual de individuos prediabéticos demuestra ser excesiva en proteínas y en grasas totales que, relacionado al consumo excesivo de ácidos grasos saturados y colesterol, puede aumentar el riesgo cardiovascular. Esto, junto a la ingestión pobre en fibras e inadecuada en algunos micronutrientes, tales como vitamina D, calcio y selenio pueden afectar el control de la glucemia de estos individuos.

Abstract: Background The diet is an important strategy for glycemic control as well as reducing/eliminating the risk of developing diabetes. Objective This article evaluated the consumption of macronutrients and micronutrients of pre-diabetic individuals from Santa Cruz do Sul, in the state of Rio Grande do Sul (RS), Brazil. Method The nutrients intake was determined in the DietWin® program and the evaluation of the prevalence of adequacy macronutrients was classified according to Guidelines of the Brazilian Society of Diabetes and the evaluation of inadequacy of the micronutrients intake by Dietary Reference Intake were used. Results The pre-diabetic individuals had excessive protein and total fat intake (28.1% and 31.6%, respectively), as well as saturated fatty acids and cholesterol (91.2% and 35.1%, respectively). The fibers intake in this population showed an inadequacy of 93.0%. Considering the micronutrients intakes, vitamin D, calcium and selenium presented an insufficiency greater than 80.0%. Conclusion The usual diet of pre-diabetic individuals showed to be excessive for proteins and total fats, which associated with excessive consumption of saturated fatty acids and cholesterol, can increase cardiovascular risk. This, coupled with inadequate consumption of some micronutrients such as vitamin D, calcium and selenium and low fiber can affect the control of glycemia in these individuals.

Resumen Introducción La alimentación es una importante estrategia para el control de la glucemia, así como para reducir/eliminar el riesgo del desarrollo de la diabetes. Objetivo Este artículo evaluó el consumo de macronutrientes y micronutrientes de individuos prediabéticos de Santa Cruz do Sul, RS, Brasil. Método El consumo de nutrientes se ha determinado en el programa DietWin® y la prevalencia de adecuación de los macronutrientes fue clasificada según la Sociedad Brasileña de Diabetes y la ingesta inadecuada de micronutrientes por la Dietary Reference Intake. Resultados Los prediabéticos presentaron consumo excesivo de proteínas y grasas totales (28,1% y 31,6%, respectivamente), así como de ácidos grasos saturados y colesterol (91,2% y 35,1%, respectivamente). La ingesta de fibras se presentó insuficiente en 93%. Considerando el consumo de micronutrientes, la vitamina D, el calcio y el selenio se presentaron con una insuficiencia de más del 80%. Conclusión La dieta habitual de individuos prediabéticos demuestra ser excesiva en proteínas y en grasas totales que, relacionado al consumo excesivo de ácidos grasos saturados y colesterol, puede aumentar el riesgo cardiovascular. Esto, junto a la ingestión pobre en fibras e inadecuada en algunos micronutrientes, tales como vitamina D, calcio y selenio pueden afectar el control de la glucemia de estos individuos.

Abstract The high consumption of sugars is linked to the intermediate hyperglycemia and impaired glucose tolerance associated with obesity, inducing the prediabetes. However, the consequences of excessive invert sugar intake on glucose metabolism and genomic stability were poorly studied. The aim of this study was to evaluate the effects of invert sugar overload (32%) in rats, analyzing changes in obesity, glucose tolerance, pancreatic/hepatic histology and primary and permanent DNA damage. After 17 weeks, the rats became obese and had an excessive abdominal fat, as well as presented impaired glucose tolerance, caused by higher sugar caloric intake. Primary DNA damage, evaluated by the comet assay, was increased in the blood, however not in the pancreas. No protein carbonylation was seen in serum. Moreover, no increase in permanent DNA damage was seen in the bone marrow, evaluated using the micronucleus test. Some rats presented liver steatosis and that the pancreatic islets were enlarged, but not significantly. In this study, invert sugar altered the glucose metabolism and induced primary DNA damage in blood, but did not cause significant damage to the pancreas or liver, and neither changes in the levels of oxidative stress or permanent DNA damage.

RESUMO Objetivo Avaliar e associar a frequência do consumo de 5 porções ao dia de frutas e hortaliças com fatores socioeconômicos e demográficos em escolares de Santa Cruz do Sul (Rio Grande do Sul), Brasil. Métodos Este foi um estudo transversal com uma amostra estratificada por conglomerados de 1.578 escolares, com idade entre 7 e 17 anos de Santa Cruz do Sul (Rio Grande do Sul), Brasil. O consumo 5 porções de frutas e hortaliças foi questionado e por conseguinte classificado em consumo irregular ou regular de frutas e hortaliças. Coletou-se também informações referentes ao sexo, a idade, condição socioeconômica, zona de domicílio, bem como informações sobre o estado nutricional dos escolares. A razão de prevalência foi calculada com nível de significância de p<0,05. Resultados O consumo de pelo menos 5 porções de frutas e hortaliças ao dia foi relatado por 40,6% dos escolares. O consumo irregular de frutas e hortaliças, em relação à recomendação, foi mais elevado entre os escolares com idade entre 10 e 12 anos (69,7%, p<0,001), entre os do sexo masculino (60,9%, p=0,077) e os residentes da zona urbana (61,8%, p<0,001). Não houve diferença significativa entre o consumo de frutas e hortaliças e a condição socioeconômica (p=0,094) nem com o estado nutricional (p=0,999). Conclusão Os escolares apresentaram consumo irregular de frutas e hortaliças em relação às recomendações de 5 porções ao dia. Entretanto, a idade e a zona de domicílio, que tiveram associação significativa em relação ao consumo de frutas e hortaliças, não mostraram estar associado a condição socioeconômica e ao estado nutricional.

ABSTRACT Objective To evaluate and associate the frequency of consumption of five daily servings of fruits and vegetables with socioeconomic and demographic factors of students in the city of Santa Cruz do Sul, Brazil. Methods A cross-sectional study with a sample stratified by clusters of 1,578 students aged 7 to 17 in the city of Santa Cruz do Sul (Rio Grande do Sul state), Brazil. Volunteers were asked whether they consumed five daily servings of fruits and vegetables. This consumption was classified as irregular or regular. Data regarding gender, age, socioeconomic status, domicile area, and information on the nutritional status of students were also collected. The prevalence ratio was calculated with a significance level of p<0.05. Results The consumption of at least five servings of fruits and vegetables per day was reported by 40.6% of students. The irregular consumption of fruits and vegetables was higher among students aged 10-12 (69.7%, p<0.001), boys (60.9%, p<0.001), and urban area residents (61.8%, p<0.001). There were no significant differences between the consumption of fruits and vegetables and the students’ socioeconomic status (p=0.094), nor with their nutritional status (p=0.999). Conclusion The students presented an irregular consumption of fruits and vegetables in relation to the recommendations of five servings per day. However, age and domicile area, which were significantly associated with the consumption of fruits and vegetables, were not associated with their socioeconomic and nutritional status.

ABSTRACT We evaluated the influence of hesperidin and vitamin C (VitC) on glycemic parameters, lipid profile, and DNA damage in male Wistar rats treated with sucrose overload. Rats were divided into six experimental groups: I-water control; II-sucrose control; III-hesperidin control; IV-VitC control; V-co-treatment of sucrose plus hesperidin; VI-co-treatment of sucrose plus VitC. We measured the levels of triglycerides, total cholesterol, HDL-c, LDL-c, fasting glucose, and glycated hemoglobin (A1C). DNA damage was evaluated in blood and brain cells using the comet assay and the micronucleus test was used to evaluate chromosomal damages in the rat bone marrow. Co-treatment with VitC, but not with hesperidin, normalized the serum glucose. No effect of co-treatments was observed on A1C. The co-treatment with VitC or hesperidin did not influence the lipid profile (p>0.05). Rats co-treated with hesperidin had a significantly lower DNA damage level in blood (p<0.05) and brain (p<0.05). Rats treated with VitC only, but not those co-treated with VitC plus sucrose, had significantly higher DNA damage in brain (p<0.05). No significant differences were observed in the results of micronucleus test (p>0.05). Hesperidin and VitC showed different effects on sucrose and DNA damage levels. While VitC lowered the serum glucose, hesperidin reduced the DNA damage.

ABSTRACT The purpose of this study was to determine the effects of the high consumption of sucrose on the levels of DNA damage in blood, hippocampus and bone marrow of rats. Male Wistar rats were treated for 4 months with sucrose (10% for 60 initial days and 34% for the following 60 days) in drinking water, and then, glycemia and glycated hemoglobin (A1C) were measured. Levels of DNA damage in blood and hippocampus were evaluated by the comet assay. The micronucleus test was used to evaluate chromosomal damages in the bone marrow. The sucrose treatment significantly increased (p<0.01) the serum glucose levels (~20%) and A1C (~60%). The level of primary DNA damage was significantly increased (p<0.05) in hippocampal cells (~60%) but not in peripheral blood leukocytes (p>0.05). Additionally, it was observed a significative increase (p<0.05) in the markers of chromosomal breaks/losses in bone marrow, as indicated by the micronucleus test. This is the first study that evaluated DNA damage induced by high sucrose concentration in the hippocampus and bone marrow of rats. Sucrose-induced DNA damage was observed in both tissues. However, the mechanism of sucrose toxicity on DNA remains unknown.

Resumo Objetivos: avaliar o desenvolvimento neuropsicomotor e a estabilidade genômica associados ao folato e ferro sanguíneos em pré-escolares. Métodos: estudo transversal, no qual avaliou-se exames bioquímicos (hemograma completo, ferritina sérica, ferro e folato), desenvolvimento neuropsicomotor (Teste Denver II) e genotoxicidade (citoma de micronúcleos em células bucais esfoliadas) de 55 crianças com 36-59 meses de idade. Para a análise dos dados, empregou-se os testes T de Student, Kruskal-Wallis e correlação de Pearson ou Spearman, com nível de significância de p<0,05. Resultados: a prevalência de anemia foi de 1,8%. Pelo teste de Denver II foram classificadas 32,7% das crianças como normais e 67,3% como suspeita de atraso. As crianças com suspeita de atraso apresentaram pequena redução no hematócrito e hemoglobina (p=0,05 e p=0,14), redução intermediária de ferro e folato (p=0,29 e p=0,23) e redução marcante de ferritina (p=0,03). Ferro e folato associaram-se significativamente com a frequência de células com lesões no DNA (p<0,05). A frequência de células binucleadas associou-se positivamente com Red Cell Distribution Width (RDW) (r=0,56; p=0,02), nas crianças sem atraso e negativamente com folato (r=-0,33; p=0,05), nas crianças com atraso. Conclusões: este estudo mostrou baixa prevalência de anemia, mas elevada taxa de crianças com suspeita de atraso neuropsicomotor, possivelmente associada com baixos níveis de ferritina. Ademais, observou-se associação entre ferro e folato com dano no DNA, o que pode ter contribuído para o atraso neuropsicomotor.

Abstract Objectives: to evaluate the neuropsychomotor development and the genomic stability associated to folate and blood iron levels in preschool children. Methods: a cross-sectional study in which evaluated the biochemical exams (complete hemogram, serum ferritin, iron and folate), neuropsychomotor development (Denver II Test) and genotoxicity (micronuclei cytome in buccal mucosa cells) of 55 children aging 36-59 months old. Student´s T test, Kruskal-Wallis and Pearson's or Spearman's correlation tests were applied with a significance level of p<0.05 for data analysis. Results: the prevalence of anemia was 1.8%. The Denver II test classified 32.7% of the children as normal and 67.3% were suspected of having a delay. The children suspected of having a delay presented a slight reduction on hemoglobin and hematocrit (p=0.05 and p=0.14), intermediate reduction on iron and folate (p=0.29 and p=0.23) and a notable reduction on ferritin (p=0.03). Folate and iron were significantly associated to the frequency of cells with DNA damages (p<0.05). The frequency of binucleated cells was positively associated to the Red Cell Distribution Width (RDW) (r=0.56; p=0.02) in children without a delay and negatively with folate (r=-0.334; p=0.047) in children with a delay. Conclusions: this study showed a low prevalence of anemia, but a high rate of children suspected of having a neuropsychomotor, possibly associated to low ferritin levels. Additionally, iron and folate were associated to DNA damage which may have contributed to the psychomotor development delay.

RESUMO Objetivo: Avaliar o efeito da vitamina C na prevenção da obesidade e da hiperglicemia, em ratos Wistar tratados com sobrecarga de açúcar invertido, para induzir o estágio de pré-diabetes. Métodos: Ratos Wistar machos (100 dias de vida e peso médio de 336,58±23,43g) foram distribuídos aleatoriamente nos grupos: (1) controle água; (2) controle açúcar invertido, recebendo 32% de açúcar invertido diluído em água; (3) controle vitamina C, recebendo vitamina C (60mg/L) diluído em água e, (4) açúcar invertido+vitamina C, tratados com vitamina C e açúcar invertido diluídos em água. Todos os animais receberam ração e água ad libitum, sendo tratados por 17 semanas. O estágio de pré-diabetes foi avaliado considerando-se obesidade (índice de massa corporal e quantidade de gordura peritoneal) e tolerância à glicose diminuída (Teste de Tolerância à Glicose Intraperitoneal, expresso pela área sob a curva) . Resultados: Os grupos vitamina C e açúcar invertido + vitamina C apresentaram redução significativa do peso (p=0,035) e da gordura visceral (p<0,001) em relação ao grupo açúcar invertido. Consequentemente, verificou-se uma diminuição do índice de massa corporal dos grupos vitamina C e açúcar invertido+vitamina C, assemelhando-se ao do grupo C no final do experimento. A vitamina C reduziu a glicemia de jejum dos animais de ambos os grupos suplementados com Vitamina C e normalizou a tolerância à glicose do grupo açúcar invertido+vitamina C, igualando-se a área sob a curva a do grupo C. Conclusão: A suplementação de vitamina C teve efeito anti-obesogênico e hipoglicemiante, mostrando-se promissora no pré-diabetes. Estudos futuros são necessários para entender os mecanismos anti-obesogênicos e anti-hiperglicemiantes da vitamina C no pré-diabetes.

ABSTRACT Objective: To evaluate whether vitamin C can help to prevent obesity and hyperglycemia in Wistar rats treated with excess invert sugar to induce prediabetes. Methods: One hundred-day-old Male Wistar rats with a mean weight of 336.58±23.43g were randomly assigned to the following groups: (1) control, receiving water (C); (2) invert sugar control, receiving a 32% watery solution of invert sugar; (3) vitamin C control, receiving a watery solution of vitamin C (60mg/L), and (4) vitamin C plus invert sugar, receiving a watery solution of vitamin C and invert sugar. All animals had access to chow and water ad libitum and were treated for 17 weeks. Prediabetes was assessed according to two criteria: obesity (based on body mass indexand peritoneal fat content) and impaired glucose tolerance (assessed by the intraperitoneal glucose tolerance test and expressed as area under the curve) . Results: Group invert sugar control gained significantly more weight (p=0.035) and visceral fat (p<0.001) than groups vitamin C control and vitamin C plus invert sugar. Consequently, groups vitamin C control and vitamin C plus invert sugar had gained as little body mass index as group C by the end of the experiment. Vitamin C decreased the fasting glycemia of both groups supplemented with vitamin C and normalized the glucose tolerance of group vitamin C plus invert sugar, whose area under the curve matched that of group C. Conclusion: Vitamin C has anti-obesogenic and glycemia-lowering effects in Wistar rats, which might be promising to prediabetics. Future studies are needed to understand the anti-obesogenic and anti-hyperglycemic mechanisms of vitamin C in prediabetes.

RESUMO Este estudo avaliou a memória de reconhecimento e os níveis de danos no DNA (sangue e hipocampo) em ratos Wistar jovens desnutridos. O experimento foi conduzido ao longo de 14 semanas com os animais divididos em grupo controle (GC, n=8) e grupo desnutrido (GD, n=12) que foi submetido a restrição calórica. O estado nutricional para desnutrição foi definido pelo Índice de Massa Corporal (IMC) de ≤0,45g/cm2 e pela pesagem dos órgãos/tecido (fígado, baço, intestino, gordura peritoneal, rim e encéfalo). O Teste de Reconhecimento de Objeto Novo avaliou a memória de reconhecimento e o Ensaio Cometa avaliou os níveis de danos no DNA. O teste t de Student, ANOVA de duas vias e a análise de correlação de Pearson foram usados e o nível de significância foi de p<0,05. O GD apresentou um menor IMC e menores pesos dos órgãos/tecido que o GC (p<0,001). Em relação à memória de curto prazo, a taxa de reconhecimento foi maior no GD (p<0,05), apenas após 4 semanas. Em relação à memória de longo prazo, novamente a taxa de reconhecimento foi maior no GD em comparação ao GC, depois de 4 semanas (p<0,001) e depois de 14 semanas (p<0,01). O GD apresentou diminuição dos níveis de danos no DNA no sangue (p<0,01) e aumento nos níveis de danos no DNA no hipocampo (p<0,01). Concluiu-se neste estudo que a desnutrição pela restrição calórica não causou prejuízo na memória de reconhecimento, porém induziu danos no DNA no hipocampo.

ABSTRACT This study evaluated the recognition memory and the levels of DNA damage (blood and hippocampus) in undernourished young Wistar rats. The experiment was conducted along 14-week with rodents divided in control group (CG, n=8) and undernourished group (UG, n=12) which was submitted to caloric restriction. Nutritional status for undernutrition was defined by Body Mass Index (BMI) ≤0.45g/cm2 and by weighting the organs/tissue (liver, spleen, intestine, peritoneal fat, kidney and encephalon). The Novel Object Recognition Test assessed recognition memory and the Comet Assay evaluated the levels of DNA damage. Student t test, 2-way ANOVA and Pearson's correlation analysis were used and the significance level was of p<0.05. The UG showed lower BMI and organ/tissue weights than CG (p<0.001). In short-term memory, the recognition rate was higher in the UG (p<0.05), only after 4 weeks. In the long-term memory, again recognition rate was higher in the UG than the CG, after 4 weeks (p<0.001) and 14 weeks (p<0.01). The UG showed decreased levels of DNA damage in the blood (p<0.01) and increased levels in the hippocampus (p<0.01). We concluded in this study that the undernutrition by caloric restriction did not cause impairment in recognition memory, however induced DNA damage in the hippocampus.